Composite

Part:BBa_K3470003:Design

Designed by: Adithi Somayaji   Group: iGEM20_MIT_MAHE   (2020-10-16)


Modified Mer operon for methylmercury poisoning


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 894
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1592
    Illegal NgoMIV site found at 3061
    Illegal NgoMIV site found at 3109
    Illegal NgoMIV site found at 3171
    Illegal NgoMIV site found at 3382
    Illegal NgoMIV site found at 3967
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 879


Design Notes

-


Source

It contains MerR and pMerT from Shigella flexneri, MerP, MerT, MerA and MerB from Serratia marcescens, MerC, MerE and constitutive promoter having no natural comparator(synthetic), SoxR, double terminator and RBS from Escherichia coli K-12, and SYFP2 from Aequorea victoria

References